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SRX21751599: GSM7774226: E0771, Malt1_PD, Rep3; Mus musculus; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 27.7M spots, 8.3G bases, 2.5Gb downloads

External Id: GSM7774226_r1
Submitted by: Tsinghua University
Study: RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)
show Abstracthide Abstract
To reveal the downstream paracrine signals from WT-Matl1 or PD-Malt1 tumor cells which responsible for the above observed macrophage phenotypes, we collected mRNA samples from Vector control-, WT-Malt1-, PD-Malt1-, V87R-Malt1-, or L88D-Malt1-expressing E0771 cancer cells, and performed RNA-seq analysis. Overall design: E0771 cells expressing either -Vector control, wild type-Malt1 (M-WT), Malt1 PD mutant (M-PD), Malt1 V87R and Malt1 L88D were lysed by TRIZOL for RNA extraction, perform reverse transcription and cDNA library for RNA-Seq analysis.
Sample: E0771, Malt1_PD, Rep3
SAMN37354728 • SRS18859109 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7774226
Instrument: HiSeq X Ten
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer's instructions. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat# E7765) was used to generate sequencing libraires from 1 μg of total RNA. All library preparations were conducted according to the manufacturer's instructions. Paired-end 150bp sequencing was performed using an Illumina NovaSeq platform.
Runs: 1 run, 27.7M spots, 8.3G bases, 2.5Gb
Run# of Spots# of BasesSizePublished
SRR2603438427,718,0888.3G2.5Gb2024-04-18

ID:
29432920

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